P-049 Verteporfin suppresses cell proliferation, survival and migration of TCam-2 Human Seminoma Cells via inhibits the YAP-TEAD complex

Abstract Study question Does verteporfin administration effect on proliferation, survival and migration of TCam-2 human seminoma cells? Summary answer Verteporfin may be a promising anticancer agent for cells via inhibition cell viability, stimulation apoptosis. What is known already Seminoma germ tumor the testis first seminoma-derived line that retains many characteristic traits seminoma. The Hippo signalling evolutionarily highly conserved pathway controls organ size, tissue homeostasis, cancer development. In mammals, consists serine/threonine kinases Mammalian Sterile 20-protein Kinase 1 2 (MST1/2), Large Tumor Suppressor Homologues (LATS1/2), Yes-Associated Protein (YAP), its transcriptional cofactor TAZ. inhibits YAP–TEAD complex, blocking proliferation survival. design, duration were treated with different concentrations (1, 5, 10, 20 40 µM) 24, 48 72 hours (h). Participants/materials, setting, methods at doses incubation times. Afterwards, protein expression proteins was determined by western blot, mRNA levels analyzed qRT-PCR localizations evaluated immunoflourescence staining. Cell determinated wound-healing scratch assay; early-late apoptosis necrosis rates flow cytometry. Main results role chance We detected μM decreased LATS1/2 p-LATS1/2 after 48h 72h treatment (p < 0.05, **p<0.01). MST1/2 p-MST1/2 significantly in groups µM h ****p<0.0001). YAP all (**p<0.01, ***p<0.001, p-YAP only (**p<0.01). TEAD4 (****p<0.0001). After treatment, no significant difference observed MST1, MST2, LATS1, LATS2 YAP1 cells, while level compared to control ***p<0.001). also showed signaling localized nucleus especially TEAD4, translocated cytoplasm depending increasing dose cells. (****p<0.0001), viability as well increased ****p<0.0001) dose-dependent manner. Limitations, reasons caution gifted Prof. Dr. Hubert Schorle (Department Developmental Pathology Institute Molecular Diagnostic Pathology, Faculty Medicine, Germany, Bonn) our laboratory. There limitation scope study. Wider implications findings time suggested relationship therapeutic target affecting survival, Trial registration number not applicable